Biomechanics Research on microRNA-223 Expression Activated the Phosphatidylinositol-3-Kinase/Protein Kinase B Pathway by Biotically Methylating the Long Noncoding RNA KCNQ1OT1

The objective of this work was to identify novel downstream targets and evaluate the clinically interpretable significance long noncoding RNA KCNQ1OT1 expression in prognosis nasopharyngeal cancer. Patient with cancer (n=46) health volunteer (n=10) were collected from Beijing Army General Hospital (Beijing, China). Nasopharyngeal line 13-9B cells bought Chinese Academy Sciences Shanghai grown Dulbecco’s modified eagle medium (Hyclone) supplemented 10 % fetal bovine serum. 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyl-2H-tetrazolium bromide assay used detect cell growth. Flow cytometric analysis apoptosis. Enzyme-linked immunosorbent Western blotting analyze for sake visual quality control. According study's findings, higher tumor tissue patients than it tissues para carcinomas. Additionally, might encourage growth quicken Besides, could also reduce activities caspase-3/8/9 cells. Importantly, reduced microRNA-223 Long induced insulin-like factor-1 receptor/phosphatidylinositol- 3-kinase/protein kinase B pathway by microRNA-223. M6A methylation enhances stability KCNQ1OT1. Biologically-inspired, nasopharynx targeting receptor phosphatidylinositol-3-kinase/protein boosted proliferation migration.